Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

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Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids.They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources.The current study aims to isolate and screen protease-producing bacterial strains from the soil and to produce protease from the bacterial co-cultures using Evaporator Cover Clip Extension solid-state fermentation (SSF).Primary screening of the protease-producing bacterial strains was carried out on skim milk agar and they were sub-cultured and preserved on the nutrient agar for further testing.Thirty-two compatibility tests of twenty-seven bacterial isolates were performed and SSF was carried out.

Afterward, absorbance was taken at 660 nm against tyrosine as standard.According to the results, the bacterial co-culture 19 showed the highest absorbance with an enzyme activity of 10.2 U/ml.The bacterial strains of the co-culture 19 were identified through morphological and biochemical tests.Bacterial strain 1 was observed as cocci and irregular, while bacterial strain 2 was bacillus and rod-shaped.

Both FIG FENNEL strains were positive for gram staining, catalase test, casein hydrolysis test and methyl red test.As for endospore staining, bacterial strain 1 was spore forming while bacterial strain 2 was a non-spore former.It was concluded that the bacterial co-culture 19 can act as a potent co-culture for protease production.Compatibility test was carried out to enhance the production of protease by utilizing cheap and readily available agro-waste products, which benefit the industry by being cost effective and the environment by being eco-friendly.Copyright (c) The Authors.